StepOnePlus is our machine for real-time/quantitative PCR.
The StepOnePlus can do both real-time runs or end-point runs.
The StepOnePlus is connected to a laptop with the StepOne software. It is also possible to run it as a stand-alone system.
The system has four filters that can detect these fluorophores:
Filter |
Dye |
emission |
1 |
FAM
SYBR Green
|
~ 520 nm |
2 |
JOE
VIC
|
~ 550 nm |
3 |
TAMRA
NED
|
~ 580 nm |
4 |
ROX |
~ 610 nm |
Extensive manuals for the following types of experiments are found in the biology lab's index file:
- Standard curve experiments
- Relative standard curve and comparative CT experiments
- Genotyping experiments
- Precence/absence experiments