Picture of StepOnePlus real-time PCR
Current status:
AVAILABLE
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StepOnePlus is our machine for real-time/quantitative PCR. 

The StepOnePlus can do both real-time runs or end-point runs.

The StepOnePlus is connected to a laptop with the StepOne software. It is also possible to run it as a stand-alone system.

 

The system has four filters that can detect these fluorophores:

Filter Dye emission
1

FAM

SYBR Green

~ 520 nm
2

JOE

VIC

~ 550 nm
3

TAMRA

NED

~ 580 nm
4 ROX ~ 610 nm

 

Extensive manuals for the following types of experiments are found in the biology lab's index file:

  • Standard curve experiments
  • Relative standard curve and comparative CT experiments
  • Genotyping experiments
  • Precence/absence experiments
Tool name:
StepOnePlus real-time PCR
Area/room:
G1-30 Bioelectronics
Category:
Chemical and Biological methods
Manufacturer:
Applied Biosystems
Model:
StepOnePlus
Tool rate:
A

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